Bovine transforming growth factors β2,TGFβ2 ELISA Kit

Bovine transforming growth factors β2,TGFβ2 ELISA Kit

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文獻(xiàn)引用 (1) 說明書下載

中文名稱：

牛轉(zhuǎn)化生長因子β2(TGFβ2)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E14209B
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
促銷：
其他：

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產(chǎn)品描述：

牛轉(zhuǎn)化生長因子β2(TGFβ2)酶聯(lián)免疫試劑盒（CSB-E14209B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TGFB2含量。TGFB2 即轉(zhuǎn)化生長因子β2，是 TGF - β超家族成員。它在胚胎發(fā)育、組織修復(fù)、免疫調(diào)節(jié)等過程中發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)其異常表達(dá)與腫瘤進(jìn)展、纖維化疾病密切相關(guān)，機(jī)制涉及細(xì)胞增殖、分化、遷移及細(xì)胞外基質(zhì)合成等調(diào)控。試劑盒檢測范圍為6.25 ng/mL-100 ng/mL，適用于動(dòng)物模型研究、疾病機(jī)制分析（如炎癥反應(yīng)、纖維化或腫瘤微環(huán)境研究）以及生物標(biāo)志物篩選等科研場景，支持從低豐度到高濃度樣本的穩(wěn)定檢測，為探索牛源生物樣本中TGFβ2的表達(dá)調(diào)控及其功能研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

TGFB2 ELISA Kit; Transforming growth factor beta-2 proprotein ELISA Kit; Milk growth factor ELISA Kit; MGF) [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
縮寫：

TGFB2
Uniprot No.：

P21214
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 ng/mL-100 ng/mL
靈敏度：

6.25 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine TGFβ2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-89
1:2	Average %	83
	Range %	80-86
1:4	Average %	97
	Range %	93-100
1:8	Average %	96
	Range %	91-101

回收率：

The recovery of bovine TGFβ2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	87-93
EDTA plasma (n=4)	94	89-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
100	0.204	0.198	0.201
50	0.356	0.358	0.357
25	0.592	0.572	0.582
12.5	1.107	1.105	1.106
6.25	2.332	2.369	2.351

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Promoting intestinal healing by exclusive enteral nutrition with TGF-beta in a mouse model of colitis. K Boumessid,Biorxiv,2023

功能：

Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.; Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
基因功能參考文獻(xiàn)：
1. Data show that TGF-beta pathways operate during ovarian fetal development, and fibrillin 3 is highly expressed at a critical stage early in developing human and bovine fetal ovaries. PMID: 21411746
亞細(xì)胞定位：

[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-2]: Secreted.
蛋白家族：

TGF-beta family
數(shù)據(jù)庫鏈接：

KEGG: bta:534069

STRING: 9913.ENSBTAP00000007053

UniGene: Bt.6438

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.