Bovine neuropeptide Y (NPY) ELISA kit

Bovine neuropeptide Y (NPY) ELISA kit

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中文名稱：

牛神經(jīng)肽Y(NPY)酶聯(lián)免疫試劑盒
貨號：

CSB-E08171b
規(guī)格：

96T/48T
價格：

￥4800/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

牛神經(jīng)肽Y(NPY)酶聯(lián)免疫試劑盒（CSB-E08171b）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的NPY含量。NPY即神經(jīng)肽Y，是一種廣泛分布于中樞和外周神經(jīng)系統(tǒng)的肽類神經(jīng)遞質(zhì)。它在調(diào)節(jié)攝食行為、能量代謝、心血管活動等方面發(fā)揮重要作用。研究機(jī)制上，主要通過與相應(yīng)受體結(jié)合，激活細(xì)胞內(nèi)信號轉(zhuǎn)導(dǎo)途徑，進(jìn)而產(chǎn)生一系列生理效應(yīng)。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品可用于科研領(lǐng)域探索NPY在牛的能量代謝調(diào)控、應(yīng)激生理響應(yīng)、神經(jīng)內(nèi)分泌網(wǎng)絡(luò)中的作用機(jī)制，例如在飼喂干預(yù)、環(huán)境壓力模型或神經(jīng)功能研究中動態(tài)監(jiān)測NPY的表達(dá)水平變化，為畜牧學(xué)、動物生理學(xué)及比較醫(yī)學(xué)研究提供可靠的工具支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

NPY ELISA Kit; Pro-neuropeptide Y [Cleaved into: Neuropeptide Y ELISA Kit; Neuropeptide tyrosine ELISA Kit; NPY); C-flanking peptide of NPY ELISA Kit; CPON)] ELISA Kit
縮寫：

NPY
Uniprot No.：

Q6RUW3
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-93
1:2	Average %	102
	Range %	98-105
1:4	Average %	90
	Range %	86-96
1:8	Average %	91
	Range %	88-97

回收率：

The recovery of bovine NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	91	87-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.604	2.490	2.547	2.444
10	2.113	2.221	2.167	2.064
5	1.657	1.711	1.684	1.581
2.5	1.114	1.056	1.085	0.982
1.25	0.712	0.687	0.700	0.597
0.625	0.413	0.428	0.421	0.318
0.312	0.278	0.260	0.269	0.166
0	0.105	0.101	0.103

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
基因功能參考文獻(xiàn)：
1. Hormonal gene expression involved in residual feed intake in dairy cows may be related to the molecular regulation of the leptin-NPY and insulin signaling pathways. PMID: 26231801
2. Data indicate that increased body weight gain during juvenile development accelerates sexual maturation in heifers, coincident with reciprocal changes in circulating concentrations of leptin and hypothalamic neuropeptide Y (NPY) release. PMID: 25326602
3. There is a strong association between putative favorable allelic variants (SNP) of neuropeptide Y, leptin, and IGF-1 genes, and residual feed intake, when animals were grazing on a high-quality, high-availability pasture. PMID: 23881687
4. The NPY SNP (NPY1) was associated with the prevalence of the animal being in calf 100 d after calving and 305-d milk yield in the first lactation. PMID: 21700051
5. NPY tended to increase serum growth hormone, which appeared to be a consequence of increased pulse amplitude. Infusion of NPY also increased CSF growth hormone-releasing hormone. PMID: 19632078
6. The effect of single nucleotide polymorphisms in 6 genes and their associations with production factors in beef cattle are reported. PMID: 17785604
7. Data show that PKA and PKC pathways are involved in the differential regulation of production of the neuropeptides (Met)enkephalin, galanin, somatostatin, NPY, and VIP. PMID: 18619673
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亞細(xì)胞定位：

Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
蛋白家族：

NPY family
數(shù)據(jù)庫鏈接：

KEGG: bta:504216

STRING: 9913.ENSBTAP00000005903

UniGene: Bt.33726

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.