Bovine interleukin 4,IL-4 ELISA Kit

產品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛白介素4(IL-4)酶聯免疫試劑盒（CSB-E12898B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL4含量。IL4是一個重要靶點。它是白細胞介素家族成員，在免疫系統中起關鍵作用，參與調節(jié)B細胞、T細胞等免疫細胞的生長和分化。研究機制上，主要聚焦其信號通路及與疾病發(fā)生發(fā)展關聯，圍繞它開發(fā)的藥物有望用于哮喘、特應性皮炎等免疫相關疾病治療。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于科研領域探究牛類感染性疾病、過敏反應或炎癥過程中IL-4的動態(tài)變化，例如通過血清/血漿樣本評估疫苗免疫效果，或利用組織勻漿分析特定器官的免疫應答機制，為畜牧養(yǎng)殖中的疾病機制研究和免疫調控提供實驗支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
縮寫：

IL4
Uniprot No.：

P30367
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	97
	Range %	92-103
1:4	Average %	90
	Range %	85-94
1:8	Average %	84
	Range %	80-88

回收率：

The recovery of bovine IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	89	84-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
基因功能參考文獻：
1. These results support pregnancy as type 2 immune response biased, with increases of IFN-gamma occurring after parturition and an increase in IL-4 production before calving. PMID: 23814356
2. IL-4 may play a role in coordinating the adrenal response to inflammatory stress. PMID: 18055157
3. T helper (Th) type 2 cell cytokine IL-4 modulates airway contraction by secreting matrix metalloproteinase-1 from the smooth muscle cells via phosphatidylinositol 3-kinase activation and changing cell-to-matrix interactions. PMID: 18322231
亞細胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
數據庫鏈接：

KEGG: bta:280824

STRING: 9913.ENSBTAP00000021215

UniGene: Bt.423