Bovine complement fragment 5a (C5a) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛補體片斷5a(C5a)酶聯免疫試劑盒（CSB-EQ027637BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C5a含量。C5a是補體系統激活后產生的重要炎性介質。其背景在于它參與多種生理與病理過程。研究機制上，C5a能與受體結合，介導炎癥細胞趨化、活化，釋放炎癥因子，在感染、自身免疫病、腫瘤等疾病中發揮作用，是潛在治療靶點。試劑盒檢測范圍為4.69 ng/mL-300 ng/mL，該產品適用于動物免疫機制研究、炎癥性疾病模型構建、疫苗效力評估等科研領域，尤其為反芻動物感染性疾病的免疫病理分析提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

C5a
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

4.69 ng/mL-300 ng/mL
靈敏度：

1.17 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-93
1:2	Average %	101
	Range %	97-104
1:4	Average %	94
	Range %	88-99
1:8	Average %	93
	Range %	89-97

回收率：

The recovery of bovine C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	90	86-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.