Bovine anti-Mullerian hormone (AMH) ELISA kit

Bovine anti-Mullerian hormone (AMH) ELISA kit

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中文名稱：

牛繆勒管抑制物質/抗繆勒管激素(AMH)酶聯免疫試劑盒
貨號：

CSB-E13406B
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

牛繆勒管抑制物質/抗繆勒管激素(AMH)酶聯免疫試劑盒（CSB-E13406B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AMH含量。抗繆勒管激素（AMH）由卵巢顆粒細胞分泌，可反映卵巢儲備功能，與生殖健康密切相關。研究機制上，AMH參與卵泡發育調控，抑制始基卵泡啟動及竇前卵泡對FSH的敏感性。臨床常檢測其水平評估卵巢功能，輔助生殖領域應用廣泛。試劑盒檢測范圍為0.5 ng/mL-200 ng/mL，適用于畜牧業中繁殖能力研究、卵巢儲備功能評估、生殖相關疾病模型構建等科研場景，組織勻漿檢測功能還可拓展至生殖器官局部AMH表達分析。為繁殖生物學、發育生物學等領域的科研工作者提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
縮寫：

AMH
Uniprot No.：

P03972
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.5 ng/mL-200 ng/mL
靈敏度：

0.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	98
	Range %	91-105
1:4	Average %	92
	Range %	88-97
1:8	Average %	97
	Range %	93-105

回收率：

The recovery of bovine AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	97	90-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
200	0.134	0.139	0.137
37.5	0.211	0.203	0.207
6.25	0.495	0.485	0.490
1.75	0.968	0.987	0.978
0.5	1.146	1.154	1.150
0	2.226	2.250	2.238

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
基因功能參考文獻：
1. Anti-Mullerian hormone inhibits activation and growth of bovine ovarian follicles in vitro and is localized to growing follicles. PMID: 28333275
2. This study showed that antral follicle counts (AFC) and AMH levels were repeatable when determined at an unknown stage of follicular growth and expected day of follicular wave emergence, but repeatability was greater for AMH than AFC. PMID: 28237349
3. The effects of castration and other surgical intervention on the blood levels of anti-Muellerian hormone, inhibin A, gonadotropins, and gonadotropin receptors in bull calves are reported. PMID: 27693012
4. Results from these studies indicate that AMH signaling plays a role in both regulating granulosa cell proliferation and preventing granulosa cells from 5- to 8-mm follicles from undergoing premature differentiation before follicle selection. PMID: 27268296
5. These findings indicate the followings: AMH mRNA levels decrease in both dominant and secondary follicles during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co-dominant follicles. PMID: 26815645
6. Measurement of AMH concentration in the plasma of cows can help to predict their capacity for embryo production in response to gonadotrophin treatment. PMID: 22935154
7. In first study to investigate the blood profile and immunohuistochemistry of anti-Mullerian hormone in bovine granulosa-theca cell tumors, the findings indicated that anti-Mullerian hormone is a novel biomarker for granulosa-theca cell tumors in cattle. PMID: 22052009
8. Regulation of anti-Mullerian hormone production in the cow. PMID: 21076084
9. Intrafollicular AMH was not a marker of cystic development in the cow, but low AMH concentrations in cysts were associated with luteinization. PMID: 18448844
10. AMH expression is modulated by androgens in bovine granulosa cells from small follicles. PMID: 19714502
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亞細胞定位：

Secreted.
蛋白家族：

TGF-beta family
數據庫鏈接：

KEGG: bta:280718

STRING: 9913.ENSBTAP00000019912

UniGene: Bt.7050

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.