Bovine Vitamin D-binding protein(GC) ELISA kit

產品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛維生素D結合蛋白(GC)酶聯免疫試劑盒（CSB-EL009306BO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GC含量。試劑盒檢測范圍為0.156 μg/mL-10 μg/mL，適用于科研領域中對牛類動物維生素D代謝通路研究、營養狀態評估、骨骼發育相關機制探索以及免疫調節功能分析，可為畜牧營養學、獸醫學及基礎醫學研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

GCVitamin D-binding protein ELISA kit; DBP ELISA kit; VDB ELISA kit; Gc-globulin ELISA kit; Group-specific component ELISA kit
縮寫：

GC
Uniprot No.：

Q3MHN5
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 μg/mL-10 μg/mL
靈敏度：

0.039 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	82-92
1:200	Average %	93
	Range %	89-97
1:400	Average %	92
	Range %	84-96
1:800	Average %	95
	Range %	90-100

回收率：

The recovery of bovine GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	103	100-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
基因功能參考文獻：
1. Associations between SNPs and the studied traits were strongest for SNPs that were located within and immediately upstream of the group-specific component (GC) gene. Our results suggest GC as the gene that underlies the QTL for clinical mastitis and milk production. PMID: 27760518
亞細胞定位：

Secreted.
蛋白家族：

ALB/AFP/VDB family
數據庫鏈接：

KEGG: bta:530076

STRING: 9913.ENSBTAP00000033386

UniGene: Bt.53584

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