Bovine Serum amyloid A,SAA ELISA Kit

Bovine Serum amyloid A,SAA ELISA Kit

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文獻(xiàn)引用 (7) 說明書下載

中文名稱：

牛血清淀粉樣蛋白A(SAA)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08592b
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

牛血清淀粉樣蛋白A(SAA)酶聯(lián)免疫試劑盒（CSB-E08592b）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SAA1含量。SAA1即血清淀粉樣蛋白 A1，它在機(jī)體天然免疫中發(fā)揮重要作用，炎癥狀態(tài)下其水平會(huì)顯著升高。研究機(jī)制方面，它涉及多種細(xì)胞信號(hào)通路，與炎癥反應(yīng)、脂質(zhì)代謝等密切相關(guān)，還可能是某些疾病的生物標(biāo)志物或潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為0.1 μg/mL-20 μg/mL，適用于畜牧獸醫(yī)研究、動(dòng)物疾病模型構(gòu)建等領(lǐng)域，可用于探究炎癥相關(guān)疾病的分子機(jī)制、評(píng)估疫苗或藥物的免疫調(diào)節(jié)效果，以及監(jiān)測(cè)飼養(yǎng)環(huán)境中牛群健康狀態(tài)，為科研人員提供穩(wěn)定可靠的實(shí)驗(yàn)數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

SAA1 ELISA Kit; SAA ELISA Kit; Serum amyloid A protein ELISA Kit; SAA) [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA)] ELISA Kit
縮寫：

SAA1
Uniprot No.：

P35541
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.1 μg/mL-20 μg/mL
靈敏度：

0.05 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	93
	Range %	89-98
1:4	Average %	95
	Range %	91-98
1:8	Average %	92
	Range %	90-95

回收率：

The recovery of bovine SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-99
EDTA plasma (n=4)	96	89-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
0	1.963	1.977	1.970
0.1	1.644	1.654	1.649
0.4	1.239	1.245	1.242
1.6	0.817	0.798	0.808
5	0.372	0.368	0.370
20	0.121	0.123	0.122

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Immunological response enhancement in cows with subclinical mastitis fed diet supplemented with Macleaya cordata MSA Khattab, L Haiqiang, T Shaoxun,Frontiers in Veterinary Science,2025
Weather Resilience in Grazing Beef Heifers with Differing Residual Feed Intake MC Londono Mendez,Thesis&Dissertation,2024
Tropical study on Clinical and Host Cell Responses of Acute Phase Proteins, Pro-Inflammatory Cytokines and Antibodies’ in Lactating Dairy Cows Vaccinated and Challenged with S. aureus IU Hambali,ResearchGate,2022
Enhancing dietary cation-anion difference reshaped the distribution of endotoxin across different biofluids and influenced inflammatory response in dairy cows … Z.Wang,Animal Feed Science and Technology,2020
Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows Hambali IU, et al,Tropical animal health and production,2018
Effect of Sodium Bicarbonate Supplementation On Subacute Ruminal Acidosis (Sara) of Milch Cattle Bipin K C.et al,Indian Journal of Applied Research,2016
Acute-phase responses in cattle infected with hydatid cysts and microbial agents. Sevimli A.et al,J Helminthol.,2015

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功能：

Major acute phase reactant. Apolipoprotein of the HDL complex.
基因功能參考文獻(xiàn)：
1. polymorphisms in the SAA2 gene are associated with milk production traits in Chinese Holstein cows PMID: 26373797
相關(guān)疾病：

Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
亞細(xì)胞定位：

Secreted.
蛋白家族：

SAA family
組織特異性：

Expressed by the liver; secreted in plasma.
數(shù)據(jù)庫鏈接：

KEGG: bta:506412

STRING: 9913.ENSBTAP00000030310

UniGene: Bt.88760

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.