Bovine Heparin-binding growth factor 2(FGF2) ELISA kit

說明書下載

中文名稱：

牛堿性成纖維細胞生長因子/肝素結(jié)合生長因子2(FGF2)酶聯(lián)免疫試劑盒
貨號：

CSB-EL008625BO
規(guī)格：

96T/48T
價格：

￥4200/￥3000
促銷：
其他：

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牛堿性成纖維細胞生長因子/肝素結(jié)合生長因子2(FGF2)酶聯(lián)免疫試劑盒（CSB-EL008625BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF2含量。FGF2（成纖維細胞生長因子2）是成纖維細胞生長因子家族的重要成員，具有促進細胞生長、分化和血管生成等功能。其研究機制涉及通過結(jié)合FGFR受體激活細胞內(nèi)信號通路，如RAS-MAPK、PI3K-Akt等，參與細胞增殖、分化、遷移和血管生成等生理過程。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于科研領域中對FGF2功能機制的研究，包括但不限于心血管疾病模型、腫瘤微環(huán)境分析、創(chuàng)傷愈合過程等研究方向，可為細胞培養(yǎng)上清液、體液樣本及組織提取物中的FGF2動態(tài)變化提供可靠檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2) [Cleaved into: Kidney-derived growth factor] ELISA Kit
縮寫：

FGF2
Uniprot No.：

P03969
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	97
	Range %	93-102
1:4	Average %	84
	Range %	81-89
1:8	Average %	88
	Range %	83-95

回收率：

The recovery of bovine FGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-104
EDTA plasma (n=4)	92	89-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.082	2.212	2.147	1.991
200	1.298	1.302	1.300	1.144
100	0.826	0.885	0.856	0.700
50	0.527	0.564	0.546	0.390
25	0.341	0.344	0.343	0.187
12.5	0.288	0.298	0.293	0.137
6.25	0.229	0.232	0.231	0.075
0	0.152	0.160	0.156

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
亞細胞定位：

Secreted. Nucleus.
蛋白家族：

Heparin-binding growth factors family
數(shù)據(jù)庫鏈接：

STRING: 9913.ENSBTAP00000007477

UniGene: Bt.66957

Bovine Heparin-binding growth factor 2(FGF2) ELISA kit

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