Bovine Deoxyribonuclease-1(DNASE1) ELISA kit

說明書下載

中文名稱：

牛脫氧核糖核酸酶1(DNASE1)酶聯免疫試劑盒
貨號：

CSB-EL007049BO
規格：

96T/48T
價格：

￥4200/￥3000
促銷：
其他：

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產品描述：

牛脫氧核糖核酸酶1(DNASE1)酶聯免疫試劑盒（CSB-EL007049BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DNASE1含量。DNASE1是重要的靶點。它是一種核酸酶，可降解DNA。背景上，其在維持細胞內環境穩定等方面有重要意義。研究機制方面，主要聚焦其降解DNA的具體過程、調控因素，以及在相關疾病如自身免疫病中因功能異常對病情發展的影響。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，可為科研領域研究牛DNASE1在細胞凋亡機制、炎癥反應或免疫調節中的功能提供可靠工具，尤其適用于畜牧病理學、動物免疫學研究中對相關生物標志物的定量分析需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

DNASE1 ELISA Kit; DNL1Deoxyribonuclease-1 ELISA Kit; EC 3.1.21.1 ELISA Kit; Deoxyribonuclease I ELISA Kit; DNase I ELISA Kit
縮寫：

DNASE1
Uniprot No.：

P00639
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.1 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine DNASE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	85
	Range %	80-89
1:40	Average %	94
	Range %	90-98
1:80	Average %	103
	Range %	99-109
1:160	Average %	97
	Range %	92-101

回收率：

The recovery of bovine DNASE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	93	89-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.399	2.416	2.408	2.273
400	2.213	2.195	2.204	2.069
200	1.813	1.741	1.777	1.642
100	1.204	1.227	1.216	1.081
50	0.683	0.713	0.698	0.563
25	0.400	0.392	0.396	0.261
12.5	0.292	0.284	0.288	0.153
0	0.131	0.138	0.135

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
基因功能參考文獻：
1. ascorbic acid has the ability to inhibit DNase I, one of the main endonucleases involved in DNA fragmentation during apoptosis. PMID: 29551685
2. the practical capability of this assay system was successfully demonstrated by its use to determine DNase I activity in bovine urine. PMID: 27570054
3. Specificity in DNA-DNase I interaction is mediated by DNA flexibility, which influences the induced-fit transitions required to form productive complexes. PMID: 19850052
4. results indicated that the backbone hydrogen bonding in the antiparallel beta-sheet formed by its N- and C-termini, rather than the side-chain interactions, is crucial for the correct protein folding PMID: 15311926
亞細胞定位：

Secreted. Zymogen granule. Nucleus envelope.
蛋白家族：

DNase I family
數據庫鏈接：

KEGG: bta:282217

STRING: 9913.ENSBTAP00000054758

UniGene: Bt.12952

Bovine Deoxyribonuclease-1(DNASE1) ELISA kit

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