Bovine Beta-Hydroxybutyric Acid,β-OHB ELISA Kit

Bovine Beta-Hydroxybutyric Acid,β-OHB ELISA Kit

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中文名稱：

牛β羥丁酸(βOHB)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E10056b
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

牛β羥丁酸(βOHB)酶聯(lián)免疫試劑盒（CSB-E10056b）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的β-OHB含量。β-OHB即β-羥基丁酸，是酮體的主要成分之一。它在人體代謝中發(fā)揮著重要作用，可作為能量底物供能。研究發(fā)現(xiàn)其能通過調(diào)節(jié)多種信號(hào)通路，參與細(xì)胞代謝、炎癥反應(yīng)、氧化應(yīng)激等過程，在神經(jīng)保護(hù)、代謝性疾病等方面研究較多。試劑盒檢測(cè)范圍為50 nmol/mL-800 nmol/mL，適用于畜牧科研領(lǐng)域，例如探究牛群能量代謝特征、營(yíng)養(yǎng)調(diào)控對(duì)酮體生成的影響，或作為代謝綜合征模型的生物標(biāo)志物檢測(cè)工具。實(shí)驗(yàn)操作兼容常規(guī)實(shí)驗(yàn)室設(shè)備，為動(dòng)物營(yíng)養(yǎng)學(xué)、獸醫(yī)學(xué)研究提供高效、穩(wěn)定的檢測(cè)方案，數(shù)據(jù)結(jié)果僅用于科學(xué)研究，不涉及其他用途。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

beta-Hydroxybutyric acid, beta-OHB
縮寫：

β-OHB
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測(cè)范圍：

50 nmol/mL-800 nmol/mL
靈敏度：

9.5 nmol/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-OHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-95
1:2	Average %	96
	Range %	91-103
1:4	Average %	98
	Range %	92-104
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of bovine β-OHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	90-103
EDTA plasma (n=4)	95	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/ml	OD1	OD2	Average
800	0.136	0.138	0.137
400	0.309	0.306	0.308
200	0.674	0.682	0.678
100	1.470	1.475	1.473
50	2.706	2.712	2.709

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Weather Resilience in Grazing Beef Heifers with Differing Residual Feed Intake MC Londono Mendez,Thesis&Dissertation,2024
Body Condition, Energy Balance and Immune Status of Periparturient Murrah Buffaloes (Bubalus bubalis) Supplemented with Inorganic Chromium Rijusmita Sarma Deka,Biological trace element research,2016
Effects of Chain Length and Saturability of Fatty Acids on Phospholipids and Proteins in Plasma Membranes of Bovine Mammary Gland Qiongxian Yan,The Journal of membrane biology,2016
Effects of a high milk intake during the pre??eaning period on nutrient metabolism and growth rate in Japanese Black cattle Matsubara A. et al,Anim Sci J,2015

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.