Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛α2-HS糖蛋白(AHSG)酶聯免疫試劑盒（CSB-EL001485BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AHSG含量。AHSG（α2-HS糖蛋白）是一種由肝臟分泌的血漿糖蛋白，與多種疾病如糖尿病、心血管疾病和結石等密切相關。其具有高度的遺傳多態性，其基因多態性與糖尿病、血管鈣化等疾病的易感性有關。AHSG在細胞過程中發揮多種作用，如促進內吞作用、調節骨的礦物質相、影響骨穩態等。試劑盒檢測范圍為125 ng/mL-8000 ng/mL，本產品可為畜牧業中牛群健康研究、營養代謝調控實驗提供數據支持，例如骨骼發育研究、炎癥相關疾病模型構建或免疫系統功能探索等科研方向，使用者可通過檢測血清/血漿樣本評估生理狀態，或分析肝、腎等組織勻漿中的AHSG表達水平。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

AHSG ELISA Kit; FETUAAlpha-2-HS-glycoprotein ELISA Kit; Asialofetuin ELISA Kit; Fetuin-A ELISA Kit
縮寫：

AHSG
Uniprot No.：

P12763
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 ng/mL-8000 ng/mL
靈敏度：

31.25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

Quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine AHSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-98
1:200	Average %	97
	Range %	93-102
1:400	Average %	102
	Range %	98-106
1:800	Average %	88
	Range %	82-96

回收率：

The recovery of bovine AHSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	104	99-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Suggested to have lymphocyte stimulating properties, lipid binding capability and to bind thyroid hormone.
基因功能參考文獻：
1. two glycoproteins, AHSG and HPX, represent a pivotal glycoprotein of the cytoprotective activity for WN1316, showing a concrete evidence for the novel glycan-independent function of serum glycoproteins in neuroprotective drug efficacy. PMID: 29016670
2. Site-specific analysis of the O-glycosylation of bovine fetuin by electron-transfer dissociation mass spectrometry. PMID: 24907489
3. These experiments revealed a second mechanism of long-term mineral ion stabilization by the fetuin-A that is complementary to the formation of calciprotein particles. PMID: 21156141
亞細胞定位：

Secreted.
蛋白家族：

Fetuin family
組織特異性：

Liver and bone.
數據庫鏈接：

KEGG: bta:280988

STRING: 9913.ENSBTAP00000000673

UniGene: Bt.23250