Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

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中文名稱：

牛α2-HS糖蛋白(AHSG)酶聯(lián)免疫試劑盒
貨號：

CSB-EL001485BO
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

牛α2-HS糖蛋白(AHSG)酶聯(lián)免疫試劑盒（CSB-EL001485BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AHSG含量。AHSG（α2-HS糖蛋白）是一種由肝臟分泌的血漿糖蛋白，與多種疾病如糖尿病、心血管疾病和結(jié)石等密切相關(guān)。其具有高度的遺傳多態(tài)性，其基因多態(tài)性與糖尿病、血管鈣化等疾病的易感性有關(guān)。AHSG在細(xì)胞過程中發(fā)揮多種作用，如促進內(nèi)吞作用、調(diào)節(jié)骨的礦物質(zhì)相、影響骨穩(wěn)態(tài)等。試劑盒檢測范圍為125 ng/mL-8000 ng/mL，本產(chǎn)品可為畜牧業(yè)中牛群健康研究、營養(yǎng)代謝調(diào)控實驗提供數(shù)據(jù)支持，例如骨骼發(fā)育研究、炎癥相關(guān)疾病模型構(gòu)建或免疫系統(tǒng)功能探索等科研方向，使用者可通過檢測血清/血漿樣本評估生理狀態(tài)，或分析肝、腎等組織勻漿中的AHSG表達(dá)水平。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

AHSG ELISA Kit; FETUAAlpha-2-HS-glycoprotein ELISA Kit; Asialofetuin ELISA Kit; Fetuin-A ELISA Kit
縮寫：

AHSG
Uniprot No.：

P12763
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 ng/mL-8000 ng/mL
靈敏度：

31.25 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

Quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine AHSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-98
1:200	Average %	97
	Range %	93-102
1:400	Average %	102
	Range %	98-106
1:800	Average %	88
	Range %	82-96

回收率：

The recovery of bovine AHSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	104	99-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
8000	0.073	0.072	0.073
4000	0.138	0.136	0.137
2000	0.255	0.242	0.249
1000	0.435	0.447	0.441
500	0.675	0.686	0.681
250	1.038	1.049	1.044
125	1.347	1.248	1.298
0	1.593	1.695	1.644

貨期：

3-5 working days

功能：

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Suggested to have lymphocyte stimulating properties, lipid binding capability and to bind thyroid hormone.
基因功能參考文獻：
1. two glycoproteins, AHSG and HPX, represent a pivotal glycoprotein of the cytoprotective activity for WN1316, showing a concrete evidence for the novel glycan-independent function of serum glycoproteins in neuroprotective drug efficacy. PMID: 29016670
2. Site-specific analysis of the O-glycosylation of bovine fetuin by electron-transfer dissociation mass spectrometry. PMID: 24907489
3. These experiments revealed a second mechanism of long-term mineral ion stabilization by the fetuin-A that is complementary to the formation of calciprotein particles. PMID: 21156141
亞細(xì)胞定位：

Secreted.
蛋白家族：

Fetuin family
組織特異性：

Liver and bone.
數(shù)據(jù)庫鏈接：

KEGG: bta:280988

STRING: 9913.ENSBTAP00000000673

UniGene: Bt.23250

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.